Vaibhav Tyagi

4 Questions 7 Answers 0 Followers

Questions related from Vaibhav Tyagi

Why my plasmid construct is not expressing after cloning?

I have been trying to express my construct for the last couple of weeks but after several attempts, it's still failing although similar parts (five inserts for golden gate assembly) have been used...

13 December 2023 4,695 8 View

How to do proper cloning calculations for my plasmid construction pcr reaction?

Hi, I have recently started my cloning experments and don't have much experience in doing ligation reactions. I have five insert parts with each having 10ng/ul concentration and i need 200ng of...

04 December 2023 1,759 4 View

Troubleshooting EforRed Chromoprotein Extraction from E. coli

I have been attempting to extract a protein expressed using IPTG induction from my BL21 culture through sonication for an extended period, but this method has repeatedly failed. Subsequently, I...

01 January 1970 4,116 0 View

Comparative Analysis of Protein Extraction Techniques: Lysozyme Versus Sonication Methods

I would like to take some advice on a better approach for my protein extraction, which is a hydrophobin protein. I use BL21 cells for my expression culture and sonication to obtain the cell-free...

01 January 1970 7,244 3 View