Vahid Bahrambeigi

5 Questions 15 Answers 0 Followers

Questions related from Vahid Bahrambeigi

Cell line data base - Melanoma B16F10 Vs B16F0?

I have two questions. There are different available cell lines to create animal models of cancers. In the case of melanoma, there are different melanoma cell lines for instance melanoma B16F10...

05 May 2013 9,521 5 View

Western blot: no visible band

I transfected mesenchymal stem cells to express mouse interferon gamma, IFN concentration estimated by abcam Elipair anti IFN is about 1000 pg/ml , I used a vial of mouse IFN from Elipair kit as...

07 July 2012 7,807 9 View

Western blot: no visible band

I transfected mesenchymal stem cells to express mouse interferon gamma, IFN concentration estimated by abcam Elipair anti IFN is about 1000 pg/ml , I used a vial of mouse IFN from Elipair kit as...

07 July 2012 3,201 3 View

Problem in miRNA quantification by realtime PCR (SYBR Green)

I have to evaluate regulation of some miRNAs in mesenchymal stem cells. I used Qiagen miRNasy mini kit for miRNA extraction (Qiazol), after miRNA extraction I used Ambion poly A tailing kit for...

07 July 2012 7,417 20 View

Questions about pIRES-hrGFP1a

Hi every body, Plasmid pIRES-hrGFP1a is a product of Stratagen. My link In this vector there is a MCS for inserting the gene before a FLAg tag. In the vector manual you will see this...

07 July 2011 5,195 2 View