Utpol Das

6 Questions 7 Answers 0 Followers

Questions related from Utpol Das

Why C6 cell line in control is giving COX-2 expression?

I'm working with C6 glial cell line and challenging with LPS, ATP and BZATP. But in the control, getting the expression of COX-2 band. What could be the reason??

06 April 2014 5,085 1 View

Is the RNA concentration(e.g 200ng/ul) too low for RT-PCR?

I got the following concentrations of RNA from C6 cells using nanodrop. I added 50ul of nuclease free water before measuring concentration. Are the samples contaminated as 260/280 ratio below 2?

15 November 2013 1,579 27 View

How does cytokine create neuroinflammation by microglial cell (activated by LPS treating) and neuroinflammation lead to depression?

Can anyone suggests me the pathway/mechanism involved in cytokine mediated neuro-inflammation (specially due to microglial activation) and neuroinflammation leading to depression (mechanism)? How...

24 September 2013 10,052 2 View

Can someone advise on protein expression?

I 've used 0.1 ml of 10% aps to prepare 10 ml separating gel, but it was not solidified again tried with .03 ml of 25% aps, but not solidified. APS was freshly made but not solidified after...

13 September 2013 2,556 15 View

Which one (DEPC/TRIzol Reagent) helps RNA to be in the supernatant part in an RNA isolation procedure and how?

During RNA isolation DEPC and TRIzol reagent is used. How do they work and how is DNA separted from RNA?

24 May 2013 6,931 9 View

What is the best way of comparing different phylogenetics trees constructed using same dataset?

04 April 2013 5,034 3 View