Ubong Akpan

4 Questions 2 Answers 0 Followers

Questions related from Ubong Akpan

Please, I want to know what is happening to my agarose.

I mixed and melted 2% agarose with dibasic buffer at pH 6.3 but it has refuse to solidify. please what could be wrong?

02 August 2017 6,038 3 View

Is it wrong to use HCl or NaoH to adjust the pH of buffers?

Does it have any effect on biological specimen?

30 July 2017 2,059 1 View

Please I want someone to help me with the issue of faint band from restriction digestion. What are the reasons for faint bands.

The constitution of digest are as follows: water - 15ul Buffer - 2.5ul pcr - 7ul enzyme - 0.5ul I have also tried several other adjustment still the same result.. Please can someone tell the right...

07 June 2017 7,561 9 View

Please someone should help me with this. I am running a restriction digestion. The bands are very faint and there are no smears. what can I do?

The digest constitution are as follows: water - 6.5 microlitre buffer- 1 microlitre enzyme - 0.5 microlitre PCR product - 2 microlitre

31 May 2017 2,095 5 View