Tuan Nguyen

4 Questions 2 Answers 0 Followers

Questions related from Tuan Nguyen

Storing DNA as fragments vs cloned constructs?

I have a set of a couple dozen DNA constructs that I plan to order for nucleosome reconstitutions, I was wondering what are the pros and cons of having them as fragments vs cloned. I don't like...

08 August 2019 6,661 5 View

Using prep cell for nucleosome purification?

I have seen lots of papers in literature using prep cell for purifying nucleosomes (from DNA and hexasome species), but I couldn't seem to find another protocol apart from that discussed by Luger...

07 July 2019 6,806 4 View

What are the best bioinfomatic tools to predict sequence-dependent nucleosome formation?

I'm looking for a bioinformatic server/software/tools previously described to measure the nucleosome-formation score of a given set of sequences. (i.e. workflow: input sequence -> nucleosome...

04 April 2019 8,876 1 View

What is the best method for elution of protein from PAGE gel?

Does anyone have experience purifying nucleosomes or high molecular complex proteins from gel? I have a small aliquot of nucleosome (180kd+) that I would like to separate from DNA (

10 October 2018 2,873 1 View