Tripti Seth

2 Questions 3 Answers 0 Followers

Questions related from Tripti Seth

I am trying to FACS sort transfected neurons from primary neuron culture but I am struggling to find a protocol to gently dissociate the grown neuron?

I want to FACS sort at DIV4. It will be great if anyone can tell me how to dissociate neurons with harming them and which media to use for sorting? Thanks a lot

09 April 2024 3,467 0 View

Can anyone help me finding a protocol for knockout a gene using CRISPR in primary neuron culture?

I have transfected neurons using electroporation before playing plasmid with Cas9 which makes a single double stranded cut. But I am not able to see any results. Is there a way I can delete the...

01 April 2024 4,430 3 View