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Questions related from Trang Vu
Hi everyone, I wonder if there is any way to convert a plasmid into a linear DNA, without using restriction enzyme to cut? I thought of using Rolling Circle Amplification, but that might be a...
02 February 2020 10,086 15 View
I am having trouble amplifying a sequence from cell-free DNA. I tested this primer before with DNA from a cell line, and it worked fine. However, when I tried PCR on human cell-free DNA, nothing...
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Hi all, Does anyone have experiences with using Circular dichroism (CD) to detect the protein binding to DNA? In my experiment, I am planning to mix DNA and protein together, to form a...
05 May 2018 7,516 3 View
Hi everyone, I'm working with a double stranded DNA sequence that has a single stranded tail. The single stranded part can form strong hairpin structures (delG ~ -8), which I need to get rid of....
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