Trang Nghiem Huyen

5 Questions 17 Answers 0 Followers

Questions related from Trang Nghiem Huyen

Can I conclude that lane 4 has contamination?

I did PCR with templates (same concentration for all templates) following this: 1. Negative control (Water) 2. pNL4-3 3. Negative control (TE) 4. pcDNA3.1 5. DuoFluo I used the 1st PCR products...

01 January 2017 1,968 13 View

Looking at the picture 1, why doesn't the lane 3 have the same 1kb band like in the lane 2?

Picture 1. The 2nd PCR products 1. Negative control 2. Plasmid 4 3. Plasmid 5 4. Plasmid 6 Picture 2. The 2nd PCR products 1. Negative control 2. Plasmid 4 3. Plasmid 5 4. Plasmid 6 I did 2...

01 January 2017 993 6 View

What differences are there among nonspecific band, contamination, primer dimers and RNA in the gel?

1. What is nonspecific band? 2. What differences are there between nonspecific band and random contamination (i.e. put template to the next tube) in the gel? 3. What differences are there between...

01 January 2017 7,528 0 View

Should I use empty plasmid to be PCR negative control? Why?

Should I use empty plasmid to be PCR negative control? And why?

01 January 2017 8,344 6 View

Why do I have many different PCR results on gel electrophoresis despite the same PCR protocol?

Today, I set up the 1st PCR with 2.0µl templates following: 1, 5: negative control (water) 2, 6: Plasmid 1 (0.2ng/µl) 3, 7: Plasmid 2 (0.2ng/µl) 4, 8: Plasmid 3 (0.2ng/µl) Primers: gag-691F and...

12 December 2016 2,783 11 View