Trang Nguyen

10 Questions 38 Answers 0 Followers

Questions related from Trang Nguyen

The DNA concentration of Flag-C1 (control) and Flag-VAV2 should be different when transfect into the cell?

I am doing western blot to detect the activation of Rac1 (Rac1-GTP). VAV2 is known to activate Rac1. I want to confirm by western blot. The length of Flag-C1 (control) is shorter than Flag-VAV2...

15 January 2016 10,124 1 View

Why do I only observe few colonies after 16-20h incubate after transformation but a bundle of colonies in the following day(36hrs incubation at 37oC)?

Recently, I do transformation in Kanamycin resistant plate and I observe a weird phenomenon. After incubation at 37oC for 16-20h of my ligation product, I only observed few colonies. However, the...

14 May 2015 4,801 11 View

Have you ever used the "Active Rac1 Pull-Down and Detection Kit" of Lifetechnologies?

I want to confirm my target protein can activate Rac1. I used Active Rac1 Pull-Down and Detection Kit" of Lifetechnologies. I over-expressed my target protein and followed the steps as indicated...

04 May 2015 7,328 2 View

Why couldn't the antibody recognize the endogenous protein?

I want to test the protein expression of a protein around 130kD but I could not detect it. When I over-expressed the protein, I can detect the protein but not the endogenous protein. I tested...

29 April 2015 8,131 17 View

What is the difference between MG132 proteasome inhibitor and protease inhibitor cocktail tablets?

I could not detect my target endogenous protein but I can detect over-expressed protein. I used Protease Inhibitor Cocktail Tablets. My friend told me that my target protein could have a high turn...

29 April 2015 7,418 4 View

Why does the mRNA level of control siRNA is lower than the target siRNA?

I do realtime PCR for a target protein after doing siRNA transfection. However, every time when I run real time PCR, the mRNA level of target protein is higher than in the control siRNA. In the...

20 April 2015 5,312 9 View

Which method can I use to figure out which proteins interact with a target protein?

I would like to know which proteins interact with the C9orf100 protein. What method should I use? I only know one method in which our target protein will be precipitated and run the gel. Then the...

16 March 2015 2,620 3 View

Can I generate siRNA that can knock down all of the family of RGL (RGL1, RGL2, and RGL3)?

I found that over-expressed RGL3 can induce cell migration. I bought siRNA RGL3 and transfected to NIH3T3 cell line. Then, I observe cell migration of depleted cell but I could not observe a...

06 January 2015 7,081 8 View

What experiments should I do to prove that GEF activates ARP2/3 complex?

I found that RGL3 activates Rac1 and Cdc42 and enhance cell migration. I want to prove that RGL3 also activates Arp2/3 complex. Which experiments should I do to prove it? I do the experiment like...

06 January 2015 8,990 4 View

How do I analyze the adhesion turnover of cells?

I transfected a protein in to cells. This protein promotes cell migration. When I co-transfected the cells with paxillinI, I can observe a decrease of paxillin in the transfected cells compared to...

20 October 2014 3,171 6 View