Tran Minh

3 Questions 9 Answers 0 Followers

Questions related from Tran Minh

Does anyone know about housekeeping genes in Lactobacilli other than 16S rRNA?

I am working with qPCR on Lactobacillus plantarum WCFS1 and there is a high expression level of 16S rRNA gene comparing to the other genes. Therefore, I am looking for the other housekeeping genes...

09 September 2018 8,565 3 View

Question about qPCR: Can I apply the same E% value when using different concentration of primers?

I have performed the standard curve for my primer for qPCR in order for relative quantitative the expression level of the gene of interested. For standard curve, final concentration of each primer...

08 August 2018 713 10 View

Which is the best method for total DNA extraction from Lactobacillus spp. for qPCR?

I'm going to extract total DNA (both gDNA and plasmid DNA) from Lactobacillus (gram positive) for qPCR. So far I found some protocols using either glass-beads or enzymatic method (only for gram...

08 August 2018 4,362 6 View