Tonmoy Chowdhury

3 Questions 10 Answers 0 Followers

Questions related from Tonmoy Chowdhury

What is the recommended approach for resolving ambiguous bases and creating a contig sequence? and can this contig sequence be submitted to NCBI?

I have obtained both the forward and reverse sequences of a PCR amplicon, which contain a few ambiguous bases and gaps. I am considering manually editing the forward sequence using the reverse...

23 April 2023 8,276 6 View

How to fix to many non specific band in PCR?

I am doing PCR with 16s RNA primer for Ecoli. Tested different thermal protocols (55, 58, 52°C annealing temp) from previous literature but no result. I got some of my samples showing desired...

18 November 2022 8,203 8 View

How to fix too many non specific band in PCR?

I am doing PCR with 16s RNA primer for Ecoli. Tested different thermal protocols (55, 58, 52°C annealing temp) from previous literature but no result. I got some of my samples showing desired...

18 November 2022 9,207 8 View