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Questions related from Toni Leigh Goldswain
At the start of this week I ran 3 x 12% gels (I need three to fit all of my groups on) that I poured myself. I used the BioRad TGS running buffer. The gel activation looked good. I transferred to...
09 September 2016 1,418 27 View
I am looking for phospho-Akt (serine 473) in rat heart tissue samples using western blots. I have done trouble shooting for weeks but still nothing. At first we thought it was our antibodies that...
06 June 2016 2,981 6 View
I want to perform a caspase-3 assay that has not been tested on heart tissue by the manufacturers, but it has been carried out successfully by researchers in a paper that I found (it is usually...
05 May 2016 2,195 6 View
Does anyone know how much tissue is needed of a mouse heart to obtain sufficient protein for western blotting? We would like to cut a mouse heart longitudinally and use one half for blots and the...
03 March 2015 8,168 8 View
Good day For my MSc I wanted to determine the levels of TNF-a released by H9c2 cells in response to treatment with Doxorubicin. I treated every day for 5 days, so after each day I collected the...
08 August 2014 8,215 19 View
Good day I was told a few weeks back that GAPDH cannot be used as a loading control in differentiated cells since its expression does not stay constant. Has anybody heard anything about this?
08 August 2014 921 8 View
