Tobias A.

6 Questions 1 Answers 0 Followers

Questions related from Tobias A.

Is partial reverse transcription of RNA possible?

Assume we are given an RNA sequence of, say, 300 bps. AACAGCUAGCUGAUCGAUGCUAGCUG....ACACUGUACG and now we want to reverse transcribe either the 5' or 3' end in vivo, but not the entire transcript,...

22 August 2017 885 2 View

What are protein structure models whose solution give a very accurate result compared to experimental data?

I am very interested in determining the protein structure from the sequence information. For this reason I am looking for models, whose solution matches the experimental data very well. Here I...

17 March 2017 8,385 3 View

What is the best way to reduce error rates in array synthesized DNA oligonucleotide libraries?

I am looking for ways to reduce the error rates of array synthesised DNA oligonucleotide libraries of, say, approx. 100 000 unique sequences with 170 bps. These have error rates of approx. 0.5%...

20 November 2016 2,581 1 View

Are there good SNP-phenotype association databases?

Currently, I am looking for databases, which associate SNPs and phenotypes (like eye colour or breast cancer risk). These should get updated regularly with regards to recent GWAS etc. Ideally,...

14 November 2016 5,408 8 View

Is it possible the cut the ends of DNA fragments to obtain sticky ends?

Assume we are given a DNA fragment like ACTTAGC....GCTCGGCA TGAATCG...CGAGCCGT so we have blunt ends. I am looking for a nuclease, that cleaves the end, such that we obtain sticky ends. Hence, the...

20 April 2016 8,303 3 View

How do I insert DNA barcodes into animal cells and how do I transmit them to the neighbouring cells?

I am currently interested in equipping animal cells in a tissue with individual barcodes. These barcodes should get amplified in the cells (not to a cell degrading extent, of course), and also...

19 April 2016 5,190 0 View