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Questions related from Tina Agarwal
I am trying to optimize the expression conditions for S. cerevisiae yeast cells, I need high expression for downstream screening, and was wondering if anyone of you has tried higher Galactose...
03 March 2018 1,423 3 View
I am trying to plot my qRT-PCR data for expression of genes in different tissues, and I am using the 2^(-deltaCt) where deltaCT= Ct(GOI)-Ct(HKG), is this way of representation correct?
01 January 2016 8,776 2 View
Does anyone have an idea of what could be the best internal control for ChIP-PCR in sorghum? I have tried using actin, however, it does not seem to be working well, as I am getting enrichment in...
10 October 2013 3,944 1 View
Does anyone have an idea if maize protoplasts could be stored? If yes, do you know the conditions and the efficiency of the protoplast after storage?
07 July 2013 2,746 1 View
I am planning to do maize protoplast assay (dual-LUC assay) with an effector construct under the estrogen inducible promoter and my question is if anyone knows when should the induction be done...
11 November 2012 2,187 6 View
Has anyone done ChIP experiments in Sorghum? I would like to know the optimum conditions say for example the formaldehyde percentage, optimum sonication conditions and the percentage of...
08 August 2012 6,716 4 View
