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Questions related from Timothy D Cavileer
Hi, I have combined two haploid Saccharomyces cerevisiae cultures (opposite mating types) in YPD and want to select only diploids without using solid media. Each haploid has a different selectable...
12 December 2018 2,240 3 View
I'm working with primary hepatocytes. Nucleofection of large 9.5kb plasmids isn't working but a 4.5kb is. Any experience on RE cutting larger plasmids into pieces then Nucleofecting? Will they be...
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