Timothy Sheets

2 Questions 14 Answers 0 Followers

Questions related from Timothy Sheets

What are the optimal culture conditions for H23 cell line in 2D and 3D?

I have a few cell clones of NIH-H23 engineered to express SpCas9. These cells grow very slowly compared to other cancer cell lines (e.g. A549) and following thaw to expand clones they detach from...

24 September 2021 9,463 0 View

Antibodies against GRB10 for Western Blot or IHC-Fr and/or IHC-P on pig tissues?

I have generated mutations in the GRB10 gene and have yet to find a commercial antibody that works on pig tissues in order to verify my mutational efficiency. I have a mouse and human Ab that work...

25 January 2018 380 3 View