Tim Brian Simon

3 Questions 2 Answers 0 Followers

Questions related from Tim Brian Simon

RNA Seq: What experiments usually accompany an mRNA Seq focused project?

Basically, I am comparing gene expression in different neuroinflammatory pathways using mRNA Seq in a transgenic (KO) mouse model. The main goal is too see what genes are compensating during a...

13 July 2018 8,474 3 View

RNA Seq: What experiments usually accompany an mRNA Seq focused project?

Basically, I am comparing gene expression in different neuroinflammatory pathways using mRNA Seq in a transgenic (KO) mouse model. The main goal is too see what genes are compensating during a...

07 July 2018 10,032 3 View

During the transfer, only half the lanes have successful transfer whereas the others do not. i.e lanes 1-6 work, 7-15 do not. Why?

Basically, when I load the same volume and sample into each well (15 wells), only the first half the lanes result in an accurate transfer, whereas the second half do not. This is clearly seen...

06 June 2018 4,984 4 View