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Questions related from Tim Lee
Hi there. I have to use the pET28a-SUMO vector to get my target protein. However, I can‘t separate it from SUMO tag. My protein was about 15 Kda big and has a about 5 value of pI. Due to the...
10 January 2024 2,939 4 View
Hi there, My protein may bind certain nucleoside like small molecules such as ATP, and currently I think my protein may have self-modified on the basis of binding these small molecules, for...
03 April 2023 9,083 0 View
Now ,I can get ubiquitin(UB) by Vector pQE-30.How can I get phosphoribosyl ubiquitin(PR-UB)? Need protocol, plz, thanks.
28 February 2023 6,813 0 View
Hi there. My protein was found to have a high nucleic acid peak when it was further purified by exclusion chromatography. How can I remove the nucleic acids when purifying my protein?
09 November 2022 3,522 4 View
Hi there, I tried to express a protein thought to be a kind of acetyltransferase by using Rosetta-receptive E. coli.The 1L bacterial solution was very thick before induction.And I added 300 ul 1M...
17 October 2022 2,204 3 View