Tim Lee

5 Questions 3 Answers 0 Followers

Questions related from Tim Lee

How to sovle the problem of protein sticking to Ni NTA/IDA columns after SUMO tag cleavage ?

Hi there. I have to use the pET28a-SUMO vector to get my target protein. However, I can‘t separate it from SUMO tag. My protein was about 15 Kda big and has a about 5 value of pI. Due to the...

10 January 2024 2,943 4 View

How to detect the self-modification of proteins?

Hi there, My protein may bind certain nucleoside like small molecules such as ATP, and currently I think my protein may have self-modified on the basis of binding these small molecules, for...

03 April 2023 9,084 0 View

How can I get 'phosphoribosyl ubiquitin(PR-UB)' from ubiquitin(UB)?

Now ,I can get ubiquitin(UB) by Vector pQE-30.How can I get phosphoribosyl ubiquitin(PR-UB)? Need protocol, plz, thanks.

28 February 2023 6,815 0 View

How to remove the nucleic acid during the protein purification?

Hi there. My protein was found to have a high nucleic acid peak when it was further purified by exclusion chromatography. How can I remove the nucleic acids when purifying my protein?

09 November 2022 3,523 4 View

Three times failed inductions of Rosetta Recipient cell with ITPG?

Hi there, I tried to express a protein thought to be a kind of acetyltransferase by using Rosetta-receptive E. coli.The 1L bacterial solution was very thick before induction.And I added 300 ul 1M...

17 October 2022 2,205 3 View