How to reduce background staining in N-acetylgalactosaminyltransferase 3 immunohistochemistry ?

I'm doing experiment with N-acetylgalactosaminyltransferase 3 (GalNacT3) on endometrial cancer ( antigen retrieve with citrat buffer pH 6, 120 degree, 3 minutes; blocking H2O2 in 5 minutes; fist...

22 March 2016 1,119 6 View