Thuvaraka Ware

2 Questions 4 Answers 0 Followers

Questions related from Thuvaraka Ware

Inactivated e.coli not stimulating?

hi my heat inactivated E.coli UTI89 are not stimulating my PBMCs - nor is my LPS. I have tried multiple samples and with several changes of reagents/ techniques. any other solutions? many thanks

27 May 2021 2,261 2 View

Unstimulated PBMCs being stimulated - what could be causing this?

My unstimulated PBMCs (thawed) show a better differentiation and cytokine profile on facs than my E.coli (heat inactivated) stimulated ones (but less than my CD3/28 stimulated positive controls)....

24 May 2021 1,567 3 View