Thu Tran

3 Questions 10 Answers 0 Followers

Questions related from Thu Tran

Strange band from S. cerevisiae genomic DNA?

Hi everyone, All of my genomic DNA purifications from S. cerevisiae have contained a distinct band over 10 kb. None of the yeast chromosomes are that small and it seems to be too specific for...

25 September 2017 8,876 7 View

Yeast colony PCR troubleshoot

Hi everyone, I'm trying to perform colony PCR on a S. cerevisiae industrial strain using Zymolyase. So far, I've used 0.05 - 0.1 U/ml Zymolyase in PBS pH 7.4 per colony, incubate at 37 C for 30 -...

18 May 2017 6,306 8 View

What software to use to call out polymorphisms from multiple sequence alignment?

Hi all, We have multiple sequence alignments from thousands of genes and we are wondering what to use to index all the point mutations/insertion/deletion sites without having to manually inspect...

27 May 2016 3,366 3 View