Thomas Førland Oftedal

3 Questions 2 Answers 0 Followers

Questions related from Thomas Førland Oftedal

Do fluorescein bind and stain/label bacterial cells?

I have a FITC-labeled peptide that is supposed to bind only to certain gram-positive cells, but they seem to stain all the bacterial cells quite effectively no matter the buffer conditions. There...

01 January 2020 1,346 3 View

How do you best ensure unbiased trypsin digestion for more reliable LFQ?

In my limited experience in proteomics it seems obvious that trypsin digestion and sample preparation is highly biased towards proteins of certain properties. Despite being the most abundant...

12 December 2019 10,032 3 View

Why do we incubate trypsin on ice prior to digestion?

A lot of protocols for in-gel trypsin digestion of proteins recommend leaving the dried gel piece to swell with trypsin solution on ice for 15-60 minutes prior to incubation at 37 C. Do anyone...

10 October 2019 1,113 1 View