Thomas Førland Oftedal

5 Questions 1 Answers 0 Followers

Questions related from Thomas Førland Oftedal

How can you determine if a fluorophore is adsorbed to a bacterial cell or localized in the cytoplasm?

I have a protein (conjugated with FITC fluorophore) that may adsorb and cover the bacterial cell surface or be taken up into the cytoplasm. Using confocal (fluorescence) microscopy, how can you...

12 May 2020 705 4 View

Can you investigate the mode of action of a receptor-mediated antimicrobial peptide by fluorescence polarization using whole cells?

I am trying to learn and understand the mechanisms involved in receptor-mediated killing of bacterial cells by some peptides. I have these peptides conjugated with a fluorophore for other...

20 April 2020 2,072 0 View

What is the mechanism of the Wehnelt cylinder on a thermionic filament?

For a conventional tungsten-filament "electron gun" used in electron microscopes they say the Wehnelt cap suppress electron emission from the filament at the sides and base, concentrating emission...

27 January 2020 8,598 4 View

Why do we incubate trypsin on ice prior to digestion?

A lot of protocols for in-gel trypsin digestion of proteins recommend leaving the dried gel piece to swell with trypsin solution on ice for 15-60 minutes prior to incubation at 37 C. Do anyone...

25 October 2019 5,944 1 View

Up/down-regulation of a subset of genes on a single operon

When reading publications doing transcriptomics studies in bacteria (microarray or qPCR on e.g. L. lactis, B. subtilis, E. coli), I often see that only a subset of genes on the same operon are...

01 January 1970 2,154 1 View