Thomas Gamage

3 Questions 7 Answers 0 Followers

Questions related from Thomas Gamage

Is it normal to see 3 bands when looking at ERK1/2 (p42/p44) in a western using mouse brain?

I've begun examining ERK phosphorylation in vivo and previously my antibody for total ERK (Sigma M5670) gives me 2 bands (ERK1/2) in my HEK293 cell line, but now using it for mouse brain samples...

22 May 2015 4,501 2 View

What should the shape of an fEPSP look like following tetanus?

So I've adoped a project examing LTP (stimulating Schaffer collaterals and recording in stratum radiatum of CA1) and am able to get apparent LTP following high frequency stimulation (four trains...

20 January 2015 5,755 8 View

How do you calculate the rise-slope for an fEPSP using Clampfit?

I'm able to calculate the slope easily by placing the cursors over the linear portion. However, often the stats list the rise-slope as "not found". Is there a certain way I need to align the...

15 January 2015 9,922 4 View