Thomas Gamage

5 Questions 9 Answers 0 Followers

Questions related from Thomas Gamage

What counting efficiency should I expect with Perkin Elmer Microbeta Trilux for S35?

We recently acquired a previously owned Microbeta Trilux 1450 but the counting efficiency is a lot less than the the Topcount we were previously using. The Microbeta's efficiency for sulfur-35 is...

21 October 2018 9,819 3 View

Is it normal to see 3 bands when looking at ERK1/2 (p42/p44) in a western using mouse brain?

I've begun examining ERK phosphorylation in vivo and previously my antibody for total ERK (Sigma M5670) gives me 2 bands (ERK1/2) in my HEK293 cell line, but now using it for mouse brain samples...

22 May 2015 4,564 2 View

What is an acceptable amount of variability in fEPSP peak and slope between sweeps in hippocampal recordings?

I'm assessing LTP by stimulating the CA3 schaffer collaterals and recording for CA1 and I'm wondering what is an acceptable amount of variability in the peak and slope between each sweep from a...

16 February 2015 7,358 7 View

What should the shape of an fEPSP look like following tetanus?

So I've adoped a project examing LTP (stimulating Schaffer collaterals and recording in stratum radiatum of CA1) and am able to get apparent LTP following high frequency stimulation (four trains...

20 January 2015 5,791 8 View

How do you calculate the rise-slope for an fEPSP using Clampfit?

I'm able to calculate the slope easily by placing the cursors over the linear portion. However, often the stats list the rise-slope as "not found". Is there a certain way I need to align the...

15 January 2015 9,966 4 View