Thilini Wickramasinghe

3 Questions 10 Answers 0 Followers

Questions related from Thilini Wickramasinghe

Contamination in A549 cell culture?

I am culturing A549 cells, I have noticed there are white color particle like things floating on the media. it was visible to the naked eye also. there was no color change in the media or there...

23 February 2018 8,498 5 View

Why there were many floating cell, when THP-1 cells treated with PMA ?

I have done THP-1 cell differentiation into macrophages. I used RPMI as a media. and PMA 30nM concentration. after 24 hours incubation there were many floating cells. what could be the reason? and...

23 January 2018 3,591 3 View

Wstern Blot membrane after exposure had dark background

Can you suggest me to get rid of this dark background. This is phosho JNK antibody (cat. no: Invitrogen 44682G) I used same antibody previously without any problem so we reorder from same. after...

01 January 1970 6,691 2 View