Theo Jareno

5 Questions 7 Answers 0 Followers

Questions related from Theo Jareno

How to generate the following in silico figures?

I'm sort of new to visualization of crystal structure so please bear with me. I'm working with Chimera as my viewer as of the moment. I'm wondering how the following extended conformation of the...

04 July 2022 7,236 1 View

Are BSA standards bought commercially stored in denatured or native form?

I loaded Quick Start Bovine Serum Albumin Standard as my negative control in my Native PAGE run. I was able to see three oligomeric states (i.e. monomer, dimer and trimer) in my BSA lane. I was...

11 March 2021 6,461 6 View

Protocol to follow when performing Western Blot from a Native PAGE?

I've recently performed a western blot for my Native PAGE. My protein of interest is around 120kDA however it formed oligomers with size up to 250kDA. I used Precast Gel Any KD when running my...

18 June 2019 2,972 9 View

Is 1M Imidazole stock solution sensitive to light?

I am planning to prepare a new stock of imidazole before I proceed to purifying my proteins. It just so happens that there's still around 250mL stock of autoclaved 1M imidazole that has been...

16 February 2019 939 1 View

What does it mean if a primer-specific to a microsatellite amplified not only the expected size but also bands of high molecular weight?

I extracted a genomic DNA from a plant leaf and proceeded with determining polymorphisms in SSR markers. I used commercial primers specific in amplifiying repetitive motifs in a specific locus for...

05 May 2018 1,673 2 View