6 Questions 7 Answers 0 Followers
Questions related from The Trung Tran
Hi all, I am doing a series of large-scale experiments to extract HLA class I peptides. After the desalting step, I elute the peptides with 50% ACN instead of 30% like most people do. The idea is...
26 February 2023 1,024 0 View
Hello, Is there any one here has some experience with protein digestion using both Glu-C and Trypsin to improve sequence coverage? I am planning on using phosphate 50mM (p.H 7-8) for Glu-C to...
11 October 2017 5,272 5 View
Does anyone here have an idea how to download all the consensus sequences of transcription factor response elements (human only)? I have tried to work with several databases and software tools. So...
01 June 2017 139 4 View
Dear fellow researchers, I am working on a project to globally determine the phosphorylation quantitative profiles of cisplatin-induced apoptosis in Jurkat T-cells. I have 4 biological replicates...
14 October 2016 2,472 3 View
Dear research gate members, I am trying to detect all the members of the BCL-2 protein family in T-cell extract. However, normal procedure in proteomics experiments did not work efficiently....
04 December 2014 1,585 3 View
Hello researchgate family, My name is Trung. At the moment, I am working on a discovery based proteomics project to identify phosphopeptides. And I am using a ziptip protocol adapted from...
15 October 2014 8,330 6 View
