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Hi researchers, I am constructing a Prism TIRF, and trying to visulize signals from Cy3 or Cy5 labeled DNA immobilixed on PEG/PEG biotin surface with low camera exposure time. However, the...
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Hello researchers, I am constructing a PrismTIRF. I have a problem defining focus and detecting signals with labeled DNA (the bead sample works fine). I have looked for some possible solutions,...
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We are using the objective-type TIRF setup to check Cy3 signals. However, there were always some weird patterns appearing in the epi mode and TIRF mode with 60X objective only when we were using...
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