Thalita Camilo da Silva

6 Questions 4 Answers 0 Followers

Questions related from Thalita Camilo da Silva

I didn't observe any bands on the gel after running the PCR products. What should I do next?

I need help with a PCR. I have the following primers mecA-F 5′AAAATCGATGGTAAAGGTTGG-3′ / mecA-R 5′-AGTTCTGGCACTACCGGATTTTGC-3′. I made an initial PCR with 12.5 of GoTaq Green Master Mix, 2x, 0.8...

06 October 2023 7,207 2 View

Would it be advisable include the Safer dye in the loading DNA buffer mixture before incorporating it into the DNA sample?

I am in the process of conducting an electrophoresis and I have a Safer dye at my disposal. However, I am uncertain whether it would be more suitable to combine the Safer dye with the 6X DNA...

31 August 2023 544 3 View

Can the boiling extraction protocol be used for fungi?

Is it possible to employ the boiling extraction protocol for fungi? I am working with the ATCC strain of Candida auris and conducting environmental research on this fungus using swabs. I would...

18 August 2023 9,337 2 View

How many months is the recommend expiration period for laboratory-prepared TAE? 3 motnhs?

I prepared TAE for running electrophoresis and would like more information about how long of an expiration period I can consider.

16 August 2023 4,771 3 View

How much time and voltage to consider for running electrophoresis?

Usually, I use a time of 120 minutes and 120 V, but I am testing and standardizing new primers and I realized that perhaps the sample might have run off the gel. I would like to know if you use...

16 August 2023 7,080 1 View

How a find AMPLICON SIZE, PRODUCT OF PCR?

I would like to know how I can find out the size of the amplicon from my primers. I have these primers: Forward: GCTCCGCAGCCTGCATGGA Reverse: ACGATGCCGCCATCCTCCT. I saw the suggestion of putting...

13 August 2023 219 12 View