Terrie Lb

3 Questions 3 Answers 0 Followers

Questions related from Terrie Lb

Why do I get a positive signal on my negative control when staining extracellular vesicles on Aldehyde/Sulfate Latex beads?

Hello fellow scientists. My protein is tagged with FLAG. I transfected HEK293T with either my plasmid expressing my protein-FLAG, the empty vector plasmid or the protein wild-type (no FLAG), for...

21 June 2022 9,409 1 View

What's the best way to detect pro-inflammatory cytokines in RAW264.7 supernatant?

Hello! I'm starting my Master's project soon, and I'm in the optimization/practice step. I'm stimulating RAW264.7 cells (in a 24 well) with LPS for 18h. I did a first experiment today: a...

22 October 2020 2,844 8 View

What went wrong with my overexpression?

Thanks a lot

27 March 2019 5,698 3 View