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Hi, Do anyone has experience in Fab expression in E coli that ended up with abnormally large pellet (~14g/L, wet weight; and smelly too) which eventually gave low yield (~0.1mg/mL). My Fabs are...
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I was preparing some negative stain EM grids for protein samples. I purified the proteins with gel filtration right before grid preparation and the traces looked very good (quite symmetric and...
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For preparing negative stain EM grids, what is the upper limit of HEPES buffer concentration that is compatible with Uranyl acetate stain? I have been using phosphotungstate and it is fine, but...
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