Tatyana Pozner

3 Questions 4 Answers 0 Followers

Questions related from Tatyana Pozner

Is pJET1.2 vector suitable for transfection?

Did anyone have a positive experience with iPSC transfection of pcr product (or even HEK cells) using pJET? Will be glad to hear if it works.

07 July 2018 3,794 2 View

What is the best way to enzymatically dissociate cultured neurons without harming the neurites?

In my experience, trypsin is too hard on neurons and destroys the axons. I am also afraid that centrifugation, and pipetting cause damage. Does anyone have experience in dissociating cultured...

01 January 2018 10,066 7 View

Detaching neurons for annexin assay

I would like to perform annexin assay on cultured neurons and I am afraid that accutase incubation, followed by weak centrifugation and resuspension might disrupt neurite membrane. What is the...

07 July 2017 1,628 2 View