Tatyana Budylin Behring

3 Questions 5 Answers 0 Followers

Questions related from Tatyana Budylin Behring

Can I use Imaris for stereology if I can calculate the volume through the surfaces function and can count cells using the spots and coloc functions?

I have confocal images with two markers that I will colocalize, and have 30 micron thick sections, but the stain penetration doesn't always take up 30 microns. Also would I need to remove the...

24 September 2015 5,022 4 View

How can I make my doublecortin (DCX) antibody penetrate more than 30% of my 30 micron brain slice?

I already tried to use increasing amounts of tween or triton x and have tried waiting 3 nights. My other antibodies penetrate through the tissue just fine, BRDU and NeuN. Thank you in...

10 August 2015 2,136 5 View

Is my DCX antibody staining specific?

I am trying to quantify BRDU/DCX positive cells, but have had bad luck with both of the antibodies working. BRDU will only work with HCl pretreatment. DCX will not work following HCl treatment,...

29 July 2015 9,026 4 View