Tariq Hussain

3 Questions 2 Answers 0 Followers

Questions related from Tariq Hussain

Can someone help me how to regulate the expression of a particular miRNA in experimental animals (mice)?

Hi, I am working on the regulation of miRNAs in-vivo models (macrophages) and its role in immune responces, but i don't know how to overexpress or downredulate some targeted miRNAs in animal model...

10 October 2017 8,848 1 View

Can some one tell me if the phosphatase inhibitor did not work how much it will effect the phosphorylated protein during making cell lysate?

I am working on MAPK pathway in mouse macrophages stimulating by live and killed bacteria. Most of the time I found maximum level of all phosphorylated proteins (p38, JNK, ERK) on WB from all...

08 August 2016 210 3 View

Hi, can some one suggest me how to transfect micor-RNA in macrophages effectively and how I can get maximum transfection rate?

I am using RAW264.7 cell line and tray to transfect with different concentration of micro-RNA (mimic & inhibitor) by using lipofectamin 2000 as a transfection reagent and check the result by...

03 March 2016 2,862 5 View