Tapiwa Nyakauru

3 Questions 2 Answers 0 Followers

Questions related from Tapiwa Nyakauru

Protein expression vector with TA cloning?

I want to clone my genes of interests in an expression vector and transform BL21 for protein expression. I was planning on using pEXP5-CT-topo-TA vector...

23 August 2021 1,072 1 View

I am getting Extra bases when I sequence a PCR product amplified with Phusion Hotstart High fidelity PCR Mastermix?

I did PCR with Taq, cloned the product and sequenced it. As it is a novel gene, I wanted to confirm the sequence using High Fidelity so I did PCR with High fidelity. Upon sequencing high fidelity...

06 December 2020 1,001 3 View

I am currently trying to detect the strains of cassava mosaic virus in Zimbabwe. im having problems in my PCR, what might be the problem?

I have tried several protocols to extract DNA from cassava leaves and im getting nice DNA. however, when i try to amplify Cassava mosaic virus DNA (CMV) I am not getting bands on my gel. I once...

26 September 2018 6,922 11 View