Tapiwa Nyakauru

5 Questions 3 Answers 0 Followers

Questions related from Tapiwa Nyakauru

Protein expression vector with TA cloning?

I want to clone my genes of interests in an expression vector and transform BL21 for protein expression. I was planning on using pEXP5-CT-topo-TA vector...

23 August 2021 1,140 1 View

I am getting Extra bases when I sequence a PCR product amplified with Phusion Hotstart High fidelity PCR Mastermix?

I did PCR with Taq, cloned the product and sequenced it. As it is a novel gene, I wanted to confirm the sequence using High Fidelity so I did PCR with High fidelity. Upon sequencing high fidelity...

06 December 2020 1,063 3 View

Can i use monocot promoters (eg rice promoter) in the transformation and expression of a foreign gene in dicot plant (eg tobacco)?

can someone educate me on this one. thanks

11 January 2019 8,793 1 View

How can i conclude that there is a presence of a certain virus using Geneious blast?

i have done DNA extraction and sequences my whole genome using minION form Oxford nanopore. to confirm the presence of a certain strain of the virus, i blasted my demultiplexed reads on Geneious...

10 December 2018 2,170 1 View

I am currently trying to detect the strains of cassava mosaic virus in Zimbabwe. im having problems in my PCR, what might be the problem?

I have tried several protocols to extract DNA from cassava leaves and im getting nice DNA. however, when i try to amplify Cassava mosaic virus DNA (CMV) I am not getting bands on my gel. I once...

26 September 2018 6,966 11 View