Tanmay Gharat

3 Questions 3 Answers 0 Followers

Questions related from Tanmay Gharat

Why is lyophilized ECM not digesting in a pepsin solution?

I am using Freytes et. al protocol to digest my lyophilized milled ECM material using pepsin dissolved in 0.01 N HCl? I even tried a higher dose of pepsin and a higher concentration of HCl but...

08 August 2017 6,020 2 View

Is anyone aware of FBS (fetal bovine serum) ELISA kits ? if not, could someone suggest sensitive assays to measure the amount of FBS in the media?

Please let me know

03 March 2017 7,996 4 View

Has anyone observed oil droplets in the suspension after digesting a cartilage tissue?

After digesting cartilage tissue in a collagenase solution, I always observe tiny oil-like droplets floating the digested suspension. Has anyone made a similar observation? Is the tissue a source...

01 January 2017 6,944 4 View