Tanja Davis

6 Questions 11 Answers 0 Followers

Questions related from Tanja Davis

Any suggestions for culturing THP-1 monocytes in DMEM?

I recently started culturing THP-1 cells. I know that these cells are normally cultured in RPMI 1640 media however, I need to switch them over to DMEM since I will be preparing conditioned media...

05 May 2019 1,538 4 View

Do I need to confirm the presence control plasmids in a stable cell line?

I am making stable cell lines by transfecting my cells with plasmids that will induce over-expression of my gene of interest. After selection and obtaining single colonies, I will be confirming...

03 March 2019 1,819 6 View

What are the appropriate control groups when using a stably transfected cancer cell to induce tumours in mice which will receive different treatments?

We would like to establish tumour-bearing mice models by using a cancer cell that have been stably transfected with a plasmid. Several different groups will be established which will receive...

11 November 2014 7,011 7 View

Can anyone help with creating a stable knockdown cancer cell line to be used for establishing tumour-bearing mouse model?

I would like to stably knockdown my GOI in the E0771 cell line (mouse breast cancer), which I will then inject into mice to establish a tumour-bearing model. However, I have never done this before...

05 May 2014 8,231 7 View

Are Biorad's stain-free gels compatible with Merck Immobilon Low Fluorescence PVDF membranes?

Has anyone used these two products together successfully? I was told I need to use a low fluorescent (LF) membrane when trying to visualize the proteins after transfer from a stain-free gel...

03 March 2014 6,629 9 View

High-molecular weight reference protein?

Does anyone know of a high-molecular weight reference protein? Preferably, it has to be larger than 250kDa, but larger than 120kDa will also do. Also, it needs to be expressed in epithelial cell...

12 December 2013 628 4 View