Tanay Bhatt

3 Questions 5 Answers 0 Followers

Questions related from Tanay Bhatt

Can I use Protein-A/G Dynabeads for MeDIP?

I wan to pull out anti-5mC IgG (raised in Mouse, monoclonal) [Antibody Clone-33D3] . Literature suggest using Anti-mouse Dynabeads, which we don't have! Can I proceed with Protein-G Dynabeads?

05 October 2016 3,960 4 View

Can I expand/passage the transfected 293T cells, which are giving good virus titre, to get more viruses in future?

I heard that passaging such line may increase the risk of recombination and can produce viable virus. However, with 3rd generation system has any one tried this for couple of passages? Will the...

15 October 2015 7,427 8 View

How to methylate/demethylate targeted DNA/gene promoter in in-vivo condition?

Promoter methylation can modify the gene expression

25 July 2013 9,488 2 View