Tahrima Huq

2 Questions 2 Answers 0 Followers

Questions related from Tahrima Huq

How to prepare a gel filtration column for protein isolation?

I'm trying to prepare a Sephadex-200 gel filtration column. I'm having issues with column packing and understanding how to equilibrate the column and what elution buffer to use. My sample is a...

27 October 2024 7,019 1 View

Can I make tris-HCl without Tris base?

I have access to tris-hydrochloride powder. Can I use that and adjust the ph using NaOH to make a solution of tris-Hcl (pH 8.5) rather than using Tris base? Thanks

19 December 2023 9,109 2 View