Taeyoon Jung

4 Questions 1 Answers 0 Followers

Questions related from Taeyoon Jung

Should I use FBS media when treating cells?

I plated HepG2 into a 96-well plate using EMEM containing 10% FBS and 0.1% antibiotic. After 24 hours, when treating with doxorubicin, should I use media that has 10% FBS or FBS free media? Also,...

12 November 2023 385 1 View

Can I leave Vortex inside fume hood?

I want to keep the vortex inside the hood used to treat cells. One of my coworkers says that it hinders the airflow. I think it would be more likely to get contaminated if I move in and out of the...

04 January 2022 3,291 2 View

Where do I buy EMEM without phenol red?

Our lab cultures HepG2 cells and we have been using low glucose DMEM. We are trying to get a new HepG2 from ATCC. It recommends using EMEM but I cannot find EMEM media without phenol red. I need...

28 September 2021 3,524 2 View

Does H9c2 cell grow after differentiation into cardiomyocyte?

I am trying to differentiate H9c2 cells into cardiomyocytes using 10 nM all-trans-retinoic acid. The cell growth seemed to be decreased after treatment with 10 nM RA. I plated H9c2 cells on T75...

01 January 1970 3,991 2 View