Syunghun Han

3 Questions 4 Answers 0 Followers

Questions related from Syunghun Han

Can I use regular objective dipped in water like water immersion objective?

Microscope is upright microscope, and the sample is microfluidic PDMS chip. I would like to have the chip submerged under water so there's a high chance that objective might be submerged in water...

25 July 2018 708 4 View

Best method for series of soft-lithography?

I'm using soft-lithography to build SU8 master on silica wafer. Currently, I am spin-coating SU8 for 50um thickness, soft bake then expose under UV for 1st layer for 50um then hard bake. Upon...

25 July 2018 1,208 2 View

Is anyone familiar with negative control amplification in PCR?

I am running PCR on my samples, and I've been getting intense amplification from negative control samples. I've ran 3 samples, (1 is target, 2 is NC and 3 is NC without primers) for PCR step. From...

03 December 2014 7,858 4 View