11 Questions 20 Answers 0 Followers
Questions related from Syeda Madiha Zahra
Hello, After scanning the available literature on memory cells i found that, as many papers have indicated now that Memory b cell pool is usually generated early at GC reaction, and later some...
23 March 2021 3,411 0 View
I'm trying to develop a stable model for B cell memory. for that, i kept mice for 2 months (~60 days) after primary immunization with OVA ~80ug OVA/Alum , and gave a boost next, and 6-7 days...
19 February 2021 1,981 2 View
i need to check the change in MBC's population, increase or decrease upon treatment of Murine B cells, extracted from spleen. recently i've used B220+ igG1+ igM- PD-L2+. i see some researchers...
27 October 2020 6,135 0 View
Hello i use h2O2 (100uM) for 24 hrs to mimic ischemia model in the cells (cardiomyocytes) . so when i do ROS measurement using DCFH-DA, do i need to add a positive control like ROSUP(solarbio...
09 April 2018 9,708 5 View
can someone share literature or reference to induce hypoxia invivo? and is there any alternative to hypoxia chamber to induce (cardiac) hypoxia in vivo (rat/mice) ?
11 July 2017 7,452 6 View
i want to use delphinidin (anthocyanidin) in rats, has anyoe worked with this and give me the dose (invivo) with treatment protocol? or provide some literature refernces?
08 July 2017 3,271 0 View
i want to induce hypoxia invivo (RAT or MICE), to check its effect on nrf2 mediated regulation of some target proteins in heart. and i want to use atleast two methods to induce hypoxia to get...
06 July 2017 7,352 6 View
i want to check the effect of a reagent/extract on lncRNA expression, and to see how will it regulate its expression (mechanism), that would it directly act on lncRNA OR EFFECT its transcription,...
28 June 2017 5,970 2 View
i am trying to knockdown a lncRNA using siRNA. with lipo2000 and Opti-mem. ..This experiment has been repeated 3-4 times in lab by different people. but now when i tried to do knockdown,, lncRNA...
16 June 2017 9,620 6 View
hello , can anyone guide me on how many cells should i seed in one well (12 well plate) to have single layer of cells, and that would be best for transfection (siRNA). i have to start from primary...
06 June 2017 6,329 6 View
can i use HL-1 cell line to study electrical remodeling in atrial fibrillation? any other suggestions are appreciated. thanks
07 May 2017 6,285 2 View
