Syed Adeel Zafar

4 Questions 7 Answers 0 Followers

Questions related from Syed Adeel Zafar

Is it necessary to remove the N terminal transmembrane domains from Bait protein while contructing vectors?? Why?

I am trying to find interaction among known proteins, I have a bait protein and 8 prey. My bait protein is little large > 1000 aa, which contains 3 transmembrane helix at N terminal. Is it...

07 July 2017 8,197 0 View

How can I be successful in making a vector construct with very large insert size of 10 kb?

Dear fellows, I am trying to insert a gene of almost 10 Kb into pCAMBIA 1305.1 plasmid (12.8 Kb) digested with respected enzymes. I have divided the gene into two fragments (S1 and S2) of 5 kb...

12 December 2016 8,597 1 View

Why I could not amplify a 2.5 Kb promoter region??

I am trying to amplify the promoter region of 2.5 Kb of a rice gene, it is highly GC rich, but I could not amplify it even after changing primers. My primer is totally 43 bp including the 15 bp...

08 August 2016 4,911 3 View

I have a question that during primer designing I got different length of forward and reverse primer of same gene. Can I use this primer for RT-PCR?

05 May 2015 4,931 10 View