Syed Rizvi

4 Questions 6 Answers 0 Followers

Questions related from Syed Rizvi

How to predict promoters from a set of ChIP-seq peaks?

Hello Bioinformaticians out there- I have performed some ChIP-seq experiments and at the end of it all, I have a bunch of intergenic regions that are enriched. I now need to predict promoters...

18 May 2021 6,269 1 View

How much spread is acceptable in relative gene expression in qPCR experiments?

Hi, I have recently done some qPCR after total RNA extraction, followed by first strand cDNA synthesis. The problem is, while one treatment is giving me closely lying values for relative gene...

21 May 2015 2,628 6 View

How can I study DNA-protein complex with AFM?

Hi, I want to study the structure of the DNA-protein complex using AFM. The problem is I don't have purified protein with me. So, as an alternative, I am planning to incubate the DNA fragment with...

18 February 2015 1,514 3 View

anti GBD and anti GAD antibodies?

Hi, I have to see some protein-protein interaction for which I am using the yeast two hybrid system. After constructing the fusion GBD and GAD constructs (in pGBD C1 and pGAD C1 vectors) I want to...

06 December 2012 823 1 View