Syafiqah Nadhirah Mukhtar

5 Questions 8 Answers 0 Followers

Questions related from Syafiqah Nadhirah Mukhtar

Why inhibition zones can be observed when performing Disk-diffusion method but the growth of tested bacteria was not inhibited when conducting MIC ?

Currently I am working on the antimicrobial effects of the lipopeptide biosurfactant on E. coli. When I conducted disk-diffusion method, inhibition zones can be observed on the E. coli...

07 June 2022 2,044 4 View

How to overcome the contamination problem when conducting fermentation using Streptomyces strain ? Could it be from the fermentation medium ?

I am currently working on the biosurfactant production by Streptomyces sp. in batch mode using shake flasks (submerged fermentation). Based on several literature, the fermentation for this strain...

14 March 2022 6,454 12 View

Why the patches of oil (carbon substrate) can still be observed in the fermentation broth after the fermentation process (Submerged fermentation) ?

My project is on the biosurfactant production by Streptomyces sp. which utilized the MSM as the fermentation medium and refined palm oil as the sole carbon source, in submerged fermentation....

18 January 2022 3,964 12 View

Which purification method for biosurfactant is the best & will yield a good result, normal phase silica gel column chromatography or the reverse one?

I am considering the purification method for biosurfactant produced by Streptomyces sp. Please take into considerations that this method will be conducted using conventional ways and not via...

21 November 2021 1,685 3 View

How to conduct silica gel column chromatography with solvent system of chloroform: methanol: n-hexane, with ratio of 1:1:1 ?

I have a task to conduct shake-flask fermentation and purify the biosurfactant produced by Streptomyces sp. From the papers that I have gone through so far, many of them suggested using the silica...

21 November 2021 9,100 10 View