Swati Megha

9 Questions 7 Answers 0 Followers

Questions related from Swati Megha

What length of long non coding RNA (lncRNA) should be used for its overexpression in plants?

Hi all, I am planning to over-express a lncRNA (under 35S) in Arabidopsis. Will the presence of 20-30 bp sequences upstream and downstream of lncRNA sequence affect its over-expression? I...

16 August 2019 6,840 1 View

Is there any online tool to make delta SNP index plots?

HI Research family, I am trying to construct delta SNP index Manhattan plots. Are there any stand-alone or online software for it! I am looking for something that doesn't involved programming....

21 May 2019 3,793 4 View

How to calculate PIC value for SSR markers?

Hello Everyone, This question has been asked a couple of times on this platform. I have been trying to calculate PIC value in excel using the formula PIC= 1-∑ (Pi)2. Unfortunately, I am getting...

21 July 2017 5,042 25 View

What should be the path length when doing enzyme assays in 96 well-microtiter plates?

Hello Everyone, Can someone please tell me path length to be used in the calculations when 96 well plate is used for assaying the activity of enzymes! I assume we dont use 1cm path length as in...

18 July 2017 6,125 7 View

Statistical analysis-t-test or regression analysis and analysis of qRT-PCR data?

Hello everyone! I have data at different time points for time course experiment (0h control and six samples collected after 1h, 2h, 3h,4h, 5h, 6h of a stress). I have a response for each time...

22 November 2016 3,940 10 View

How can I normalize the sequencing data in two different libraries?

Hi Everyone If I have data from four miRNA libraries. One of the libraries have 4 times more reads than other three libraries. How will the data be normalized among different libraries? People use...

06 March 2015 7,787 7 View

Can housekeeping genes (actin, GADH,UBC9 etc.) be used as an endogenous control for plant miRNA qPCR?

Hi Everyone I have tried using U6 and 5.8sRNA as endogenous control. Although the expression of both these remain constant in different conditions, the Ct values for U6 are quite high (30 or so)...

05 November 2014 900 4 View

Can Real time kinetic analysis kits available in the market serve the purpose of determining the ribonuclease activity of a plant protein?

I am planning to determine the RNase activity of a protein (provided it has this activity!). Will it be sufficient to determine RNase activity through Real time kinetic analysis kits available in...

15 December 2012 4,791 1 View

How well can the gene expression measured by qRT be correlated with the protein levels?

A gap between the mRNA and protein levels exists because of various levels of regulation. So, exactly how well can the gene expression measured by qRT be correlated with the protein levels?

07 December 2012 2,897 36 View