Swati Garg

4 Questions 7 Answers 0 Followers

Questions related from Swati Garg

Can I desalt the protein hydrolysate?

I am hydrolyzing my proteins with 6 N HCl to peptides, followed by neutralization with 2 N NaOH. Can anyone suggest the best way to remove the salts without removing the peptides? Since my...

27 November 2014 6,722 3 View

Can someone suggest how to achieve 5 % Carbon dioxide level without CO2 incubator for bacterial culture?

Our lab does not have a dedicated CO2 incubator for bacterial work. If someone could suggest whether Anaerobic kits will be able to achieve this ? If yes, How to check the level of percentage CO2...

02 September 2014 3,376 3 View

What is the reason for baseline drift while running peptide samples on RP HPLC?

When running peptide standard a stable baseline was observed but my samples are giving a very significant baseline drift (wavy)? To be precise protein samples were digested in 6N HCl and were...

12 March 2014 2,031 1 View

How to get rid of 6N HCl from protein sample?

Hydrolysed protein is to be analysed on HPLC so want to get rid of 6N HCL from the sample before loading it onto column.

23 October 2013 9,716 12 View