Swarnali Das

4 Questions 3 Answers 0 Followers

Questions related from Swarnali Das

Facing problem in ELISA with Pnpp substrate .How to fix it?

I am doing Elisa using Alkaline Phosphatase and Pnpp. Pnpp is in powder form, I have made pnpp solution in a concentration of 10mg/10ml using phosphate buffer.. But the problem is I am not getting...

31 August 2023 1,143 2 View

How can I dissolve extracted protein pellet in buffer?

I am using Tris phenol for extraction of protein from a plant sample...at the end of the extraction process I am unable to dissolve the obtained pellet in buffer(tris hcl) by using vortex. But I...

27 November 2022 1,765 1 View

Why extracted protein sample treated with UPPA turns yellowish even after adding gel loading buffer containing bromophenol blue in it?

After extracting protein from the sample using phenol in the extraction protocol..I have used UPPA 1 & 2. But the problem is the pellet obtained with UPPA treatment turns yellowish after...

20 November 2022 5,275 2 View

I have extracted protein from a pollen sample using Tris-Phenol method ,unable to get protein bands in SDS PAGE..How can I troubleshoot the problem?

I have extracted the protein sample using Tris phenol method, and after extraction I have resuspended the sample in Tris Hcl...The extracted protein sample have a concentration of 10ug/ml through...

09 November 2022 8,468 2 View