Swarali Paradkar

3 Questions 2 Answers 0 Followers

Questions related from Swarali Paradkar

Are there any methods for genotyping two alleles, together?

I am investigating the genotypes of embryos from heterozygous incrosses for a double mutation. Usually, I conduct PCR and KASP for the mutation of each allele respectively. Is there a method...

08 August 2016 9,453 1 View

Is there a way to increase the speed of staining, in in situ hybridisation using anti-DIG-AP antibody?

I am certain that the RNA that we are using for colorimetric ISH has not degraded. Some of the fish have started showing faint staining, but they haven't properly stained and it has been an hour...

20 July 2016 2,920 3 View

Why do we use SV2 and Tubulin staining in the brain?

I know it's to measure synaptic density and transport efficiency respectively, but could someone provide a more in-depth answer?I want to know why it's better than other potential markers, etc.

15 June 2016 6,868 3 View