qPCR primer efficiency question?

04 May 2019 4,333 6 View

Analysis of a cleavage site of a protein (shedding)?

I ask for a colleague and hope you can help her. She overexpressed a human protein in a bacterium and then she made a shedding experiment (enzyme which cleaves the protein). Now she wants to...

06 July 2013 9,546 23 View

Detection of old and new cells - can anyone help?

Does anybody know a method to differentiate between cells before and after cell division? We plan a migration assay and therefore we plan the following project: 1. First we seed out HUVEC cells...

06 July 2013 6,305 11 View

Why only use Alanine and Glutamine to remove N-glycosylation sites?

I asked for a colleague of my. She wants to remove a N-glycosylation site with mutagenesis. Now she read many literature as she told me and now wonders why researchers only mutated this sites...

06 July 2013 8,475 7 View

Problems with Western Blot

A colleague prepared Western blot from a protein which should normally appear only as one band on the Western blot Membrane. The concentration of the protein is low, BCA test negative. What she...

04 May 2013 6,453 11 View

Halflife of a protein / Biotinylation basic questions

I tried to research the halflife of a protein. This surface protein is expressed continuously by cells. So an idea is the biotinylation of the protein in cell culture. I am absolutely new in this...

10 November 2012 8,723 13 View

The expression of proteins

At the moment, I treat cells with a drug to test the effect of the drug for an expression profile of transcription factors. In low concentrations of the drug, the expression of the transcription...

10 November 2012 9,272 9 View

Problem with A549 cells

Hello, I have A549 cells in culture. Normally they attached really nice on normal flask, T25 or T75 without coating the flask. Now I freeze some of these cells and after thawing they don't...

06 July 2012 1,433 5 View

Problem A549 cells

Hello, I have A549 cells in culture. Normally they attached really nice on normal flask, T25 or T75 without coating the flask. Now I freeze some of these cells and after thawing they don't...

06 July 2012 7,078 9 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

What is the most important parameter of a research work, the impact factor or the h index?

The impact factor is a measure of the importance of a journal based on the number of citations received in a year. ... Articles published in a journal with a high impact factor do not all receive...

24 February 2021 7,481 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the original citation for the commonly used activity factors for converting basal metabolic rate to total caloric need?

I see the same activity factor (multipliers) in research papers and websites, but I cannot find where they come from. Does anyone know where these originated? I am looking for an original...

18 February 2021 7,635 2 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View